Mesozooplankton from Ría de Vigo (NW Spain) and its adjacent shelf between 1995 and 2003

The mesozooplankton (200 μm to 2 mm) community from monthly samples collected at two stations in Ría de Vigo (39 m) and its adjacent shelf (97 m) between 1995-2003 is described. The mesozooplankton was dominated by copepods. Inside the Ría, meroplankton (larvae of gasteropods, lamelibranchiae, echinodermate and cirripids nauplii) and other holoplanktonic organisms, such as apendicularia, cladocera, siphonophora and medusae, were also well represented. The copepod group inside the Ría was dominated by Acartia clausi, juveniles of Calanus helgolandicus, Paracalanus parvus, Pseudocalanus elongatus, Temora longicornis, Oithona plumifera, Oncaea media, Euterpina acutifrons and copepodites of Centropages spp. In the offshore station, dominant copepods species were the same than inshore together with other oceanic species, such as Paraeuchaeta hebes, Calanoides carinatus and Aetideus armatus. Biomass values within Ría de Vigo ranged between 2 and 151 mg dry weight /m3 and between 2 and 314 mg dry weight /m3 in the offshore station. Abundance ranged between 149 ind/m in January 1995 and 41814 ind/m3 in August of 2003 in the inner station, and between 22 ind/m3 in January 1996 and 93410 ind/m3 in May 1999 in the outer station. The most outstanding events were the high levels of the Mediterranean copepod Temora stylifera, usually rare in Galician waters recorded in the area in 1997 and 1998, and the increased levels of cirripid larvae observed inside the Ría

Phosphoinositide 3–kinase γ participates in T cell receptor–induced T cell activation

Class I phosphoinositide 3–kinases (PI3Ks) constitute a family of enzymes that generates 3-phosphorylated polyphosphoinositides at the cell membrane after stimulation of protein tyrosine (Tyr) kinase–associated receptors or G protein–coupled receptors (GPCRs). The class I PI3Ks are divided into two types: class IA p85/p110 heterodimers, which are activated by Tyr kinases, and the class IB p110γ isoform, which is activated by GPCR. Although the T cell receptor (TCR) is a protein Tyr kinase–associated receptor, p110γ deletion affects TCR-induced T cell stimulation. We examined whether the TCR activates p110γ, as well as the consequences of interfering with p110γ expression or function for T cell activation. We found that after TCR ligation, p110γ interacts with Gαq/11, lymphocyte-specific Tyr kinase, and ζ-associated protein. TCR stimulation activates p110γ, which affects 3-phosphorylated polyphosphoinositide levels at the immunological synapse. We show that TCR-stimulated p110γ controls RAS-related C3 botulinum substrate 1 activity, F-actin polarization, and the interaction between T cells and antigen-presenting cells, illustrating a crucial role for p110γ in TCR-induced T cell activation

Dynamic redistribution of raft domains as an organizing platform for signaling during cell chemotaxis

Spatially restricted activation of signaling molecules governs critical aspects of cell migration; the mechanism by which this is achieved nonetheless remains unknown. Using time-lapse confocal microscopy, we analyzed dynamic redistribution of lipid rafts in chemoattractant-stimulated leukocytes expressing glycosyl phosphatidylinositol–anchored green fluorescent protein (GFP-GPI). Chemoattractants induced persistent GFP-GPI redistribution to the leading edge raft (L raft) and uropod rafts of Jurkat, HL60, and dimethyl sulfoxide–differentiated HL60 cells in a pertussis toxin–sensitive, actin-dependent manner. A transmembrane, nonraft GFP protein was distributed homogeneously in moving cells. A GFP-CCR5 chimera, which partitions in L rafts, accumulated at the leading edge, and CCR5 redistribution coincided with recruitment and activation of phosphatidylinositol-3 kinase γ in L rafts in polarized, moving cells. Membrane cholesterol depletion impeded raft redistribution and asymmetric recruitment of PI3K to the cell side facing the chemoattractant source. This is the first direct evidence that lipid rafts order spatial signaling in moving mammalian cells, by concentrating the gradient sensing machinery at the leading edge

Brief Results of a Trawl Experimental Survey at NW Atlantic

An experimental survey was carried out in NAFO Regulatory Area (Div. 6EFGH and 4XWVs) and adjacent international Southern waters during last Quarter of year 2004, with one polyvalent Spanish trawler, using "Gloria" pelagic gear and bottom gear, with the aim to obtain data on distribution and biology on pelagic and demersal species in non-habitual grounds for the Spanish fleet. A scientific observer was on board to collect the information on fishing activity (effort, depth, etc.) and biological data (length distributions, length-weight relationships, etc.). A total of 119 hauls were carried out. Samplings were conducted in a wide geographical and bathymetrical range. During the survey, Alfonsino (Beryx splendens) was the main caught species

Blocking of HIV-1 Infection by Targeting CD4 to Nonraft Membrane Domains

Human immunodeficiency virus (HIV)-1 infection depends on multiple lateral interactions between the viral envelope and host cell receptors. Previous studies have suggested that these interactions are possible because HIV-1 receptors CD4, CXCR4, and CCR5 partition in cholesterol-enriched membrane raft domains. We generated CD4 partitioning mutants by substituting or deleting CD4 transmembrane and cytoplasmic domains and the CD4 ectodomain was unaltered. We report that all CD4 mutants that retain raft partitioning mediate HIV-1 entry and CD4-induced Lck activation independently of their transmembrane and cytoplasmic domains. Conversely, CD4 ectodomain targeting to a nonraft membrane fraction results in a CD4 receptor with severely diminished capacity to mediate Lck activation or HIV-1 entry, although this mutant binds gp120 as well as CD4wt. In addition, the nonraft CD4 mutant inhibits HIV-1 X4 and R5 entry in a CD4+ cell line. These results not only indicate that HIV-1 exploits host membrane raft domains as cell entry sites, but also suggest new strategies for preventing HIV-1 infection

Report of the Age Calibration Exercise Analysis for Anchovy in Division 9a (IBERAS survey 2018)

In November 2018, a new acoustic survey (IBERAS) coordinated by IEO and IPMA was carried out in order to estimate the strength of sardine and anchovy recruitment in the Atlantic waters of the Iberian Peninsula (ICES Division 9a) and to map its distribution area. As well as determine the main biological characteristics of these species in the area. In January-February 2019, an otolith reading exercise was carried out on the anchovy from the survey to determine its age, with the objective of calibrating the age readings among the anchovy readers of the IEO and the IPMA, and estimating the accuracy and discrepancies in the determination of anchovy age among these readers. As well as, to obtain the age length keys of the surve

PI3K p110δ is expressed by gp38(-)CD31(+) and gp38(+)CD31(+) spleen stromal cells and regulates their CCL19, CCL21, and LTβR mRNA levels.

The role of p110δ PI3K in lymphoid cells has been studied extensively, showing its importance in immune cell differentiation, activation and development. Altered T cell localization in p110δ-deficient mouse spleen suggested a role for p110δ in non-hematopoietic stromal cells, which maintain hematopoietic cell segregation. We tested this hypothesis using p110δ(WT/WT) mouse bone marrow to reconstitute lethally irradiated p110δ(WT/WT) or p110δ(D910A/D910A) (which express catalytically inactive p110δ) recipients, and studied localization, number and percentage of hematopoietic cell subsets in spleen and lymph nodes, in homeostatic conditions and after antigen stimulation. These analyses showed diffuse T cell areas in p110δ(D910A/D910A) and in reconstituted p110δ(D910A/D910A) mice in homeostatic conditions. In these mice, spleen CD4(+) and CD8(+) T cell numbers did not increase in response to antigen, suggesting that a p110δ(D910A/D910A) stroma defect impedes correct T cell response. FACS analysis of spleen stromal cell populations showed a decrease in the percentage of gp38(-)CD31(+) cells in p110δ(D910A/D910A) mice. qRT-PCR studies detected p110δ mRNA expression in p110δ(WT/WT) spleen gp38(-)CD31(+) and gp38(+)CD31(+) subsets, which was reduced in p110δ(D910A/D910A) spleen. Lack of p110δ activity in these cell populations correlated with lower LTβR, CCL19 and CCL21 mRNA levels; these molecules participate in T cell localization to specific spleen areas. Our results could explain the lower T cell numbers and more diffuse T cell areas found in p110δ(D910A/D910A) mouse spleen, as well as the lower T cell expansion after antigen stimulation in p110δ(D910A/D910A) compared with p110δ(WT/WT) mice

Role of the Pi3k Regulatory Subunit in the Control of Actin Organization and Cell Migration

Cell migration represents an important cellular response that utilizes cytoskeletal reorganization as its driving force. Here, we describe a new signaling cascade linking PDGF receptor stimulation to actin rearrangements and cell migration. We demonstrate that PDGF activates Cdc42 and its downstream effector N-WASP to mediate filopodia formation, actin stress fiber disassembly, and a reduction in focal adhesion complexes. Induction of the Cdc42 pathway is independent of phosphoinositide 3-kinase (PI3K) enzymatic activity, but it is dependent on the p85α regulatory subunit of PI3K. Finally, data are provided showing that activation of this pathway is required for PDGF-induced cell migration on collagen. These observations show the essential role of the PI3K regulatory subunit p85α in controlling PDGF receptor–induced cytoskeletal changes and cell migration, illustrating a novel signaling pathway that links receptor stimulation at the cell membrane with actin dynamics